POLYMORPHISM OF LIPID METABOLISM GENES IN TATARSTAN-TYPE COWS
DOI 10.12737/2782-490X-2024-57-62 УДК 636.082:577.21
Keywords:
cow, PCR, DNA, polymor phism, genotype, gene OLR1, OLR1, DGAT1, LEPAbstract
The pur pose of the research was to study the allelic polymor phism of lipid metabolism genes (OLR1,
DGAT1 and LEP) in Tatarstan type cows. The object of the study was 79 first-calf heifers of the Kholmogory breed of the
Tatarstan type, kept in the agricultural production complex “Agrofirm Rassvet” in the Kukmorsky district of the Republic
of Tatarstan. As a result of molecular genetic studies (PCR-RFLP and AS-PCR), the animals were divided into groups
taking into account their genotype at the loci of the low-density lipoprotein receptor (OLR1), diacylglycerol-Oacyltransferase (DGAT1) and leptin (LEP) genes. Animals with different genotypes of the studied genes were further divided into groups taking into account their linear affiliation. The studied sample of first-calf heifers consisted of individuals belonging to two leading genealogical lines of the Holstein breed, namely: Wis Ideal 933122 and Reflection Sovering
198998. In general, the studies showed that in the herd of first-calf heifers of the Tatarstan type, predominated allele C
(0.73) and genotype CC 49.4% for OLR1 gene; allele A (0.77) and genotype AA 57.0% for DGAT1 gene; allele C (0.59)
and genotype CT 57.0% for LEP gene. In the sample under study, the genetic balance is not shifted for any of the studied
genes. In cows, the frequency of occurrence of alleles of the OLR1, DGAT1 and LEP genes, depending on linear affiliation (W. Ideal and R. Sovering), the trend remains. Similar data were obtained that, taking into account belonging to the
Holstein breed, the highest occurrence of alleles in first-calf heifers: C (0.68-0.81) for OLR1 gene, A (0.74-0.83) for
DGAT1 gene and C (0.57 -0.60) for LEP gene, respectively.
For citation: Lamara M., Akhmetov T.M., Shaidullin R.R., Tyulkin S.V., Zarubezhnova D.V. Polymorphism of
lipid metabolism genes in Tatarstan-type cows. Agrobiotechnologies and digital farming. 2024; №1 (9): 57-62
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